molecule p selectin antagonist  (MedChemExpress)

Journal: International Journal of Molecular Sciences

Article Title: Platelets Boost Recruitment of CD133 + Bone Marrow Stem Cells to Endothelium and the Rodent Liver—The Role of P-Selectin/PSGL-1 Interactions

doi: 10.3390/ijms21176431

Figure Lengend Snippet: P-selectin/PSGL-1 dependent platelet interactions with CD133 + BMSC promote adhesion to human micro-EC under shear stress. Adherence of CD133 + BMSC to human micro endothelial cells (HMEC-1) co-incubated with human platelet rich plasma (hPRP) was tested by pairs under different conditions: control and treatment at a time. ( a ) Increased CD133 + BMSC adherence with hPRP when compared to platelet poor plasma (hPPP). ( b , c ) Both Pre-incubation of platelets with P-selectin-inhibitor KF38789 and CD133 + BMSC with PSGL-1 antagonist IM2090 revealed a reduction of adherence of CD133 + BMSC. ( d – f ): Co-incubation with PECAM-1-blocking antibody mPECAM-1.3 IgG (anti-PECAM-1), recombinant soluble human PECAM-1 (rhsPECAM-1) and CXCR4-inhibitor for SDF-1 interaction AMD3100 respectively lacked a modulating effect on CD133 + BMSC for adherence to HMEC-1. Paired t -test: * p < 0.05; ** p < 0.01; + p = 0.067; n.s. p > 0.1.

Article Snippet: In human experiments, we performed different blocking experiments by pre-incubation of different cell types with inhibitors or blocking antibodies: 20 min pre-incubation of platelets with the selective P-selectin antagonist small-molecule KF38789 (Tocris, Bio-Techne GmbH, Wiesbaden, Germany; 10 μM) [ ] at room temperature, 10 min pre-incubation of CD133 + BMSC with 10 μg/mL PSGL-1 antagonist for SDF-1 interaction IM2090 (Beckman Coulter, Krefeld, Germany; Clone 3E2-25-5-PL1) at 37 °C, 20 min pre-incubation of HMEC-1 with 10 μg/mL PECAM-1 blocking antibody mPECAM-1.3 IgG (kindly provided by Prof. P. Newman, Blood Research Institute, Milwaukee, WI, USA) at 37 °C.

Techniques: Shear, Incubation, Clinical Proteomics, Control, Blocking Assay, Recombinant